试剂盒 > 酶联免疫试剂盒 > 绵羊免疫球蛋白G(IgG)ELISA Kit
    • ELISA Kit
    • 绵羊免疫球蛋白G(IgG)ELISA Kit
  • 产品类型:
    ELISA Kit
  • 产品名称:

    绵羊免疫球蛋白G(IgG)ELISA Kit

  • 英文名称:

    Sheep Immunoglobulin G,IgG ELISA Kit

  • 货号:
    CSB-E14400Sh
  • 规格:
    96T
  • 中文价格:
    4200
  • 种属:
    Ovis aries (Sheep)
  • 其他种属:
  • 待测物名称:
    Immunoglobulin G,IgG
  • 缩写:
    IgG
  • 检测范围:
    15 μg/mL-240 μg/mL
  • 灵敏度:
    7.5 μg/mL
  • 反应时间:
    1-5h
  • 所需样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 下载:
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/ml OD1 OD2 Average    
    240 0.182 0.185 0.184    
    120 0.337 0.341 0.339    
    60 0.556 0.564 0.560    
    30 0.832 0.841 0.837    
    15 1.297 1.313 1.305    
    0 2.207 2.215 2.211    
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of sheep IgG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:100 Average % 94  
    Range % 88-97  
    1:200 Average % 96  
    Range % 91-100  
    1:400 Average % 98  
    Range % 95-106  
    1:800 Average % 93  
    Range % 87-98  
  • 回收率:
    The recovery of sheep IgG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 93 87-100  
    EDTA plasma (n=4) 95 92-98  
                 
                 
  • 定性/定量:
    quantitative
  • 研究领域:
    Immunology
  • 引用文献:
    1. , Aram Saadei Khalkhane et al,Global Veterinaria,2013
    2. , Sibel Erdo?an.et al,Revista Brasileira de Zootecnia,Feb
    3. , Gustavo Toscan.et al,SEMIN-CIENC AGRAR,5 May
    4. , A.S.Morsy.et al,Livestock Science ,Feb 22
    5. , ForoughAtaollahi.et al,Livestock Science,Octobert 5